Jellyfin Media Player encoding while direct playing?

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Could you provide ffprobe of the media file as well as recreating the issue and grabbing the ffmpeg logs from that period? You can check if any transcoding, remuxing, or audio transcoding is happening by playing the media and looking at the server dashboard. Clicking the info icon will tell you what is happening. Knowing the media information offers more insight into what might be happening, having the logs explicitly tells us.

Direct playing doesn't mean you're on the same device, by the way. It means the client (JMP in this case) is entirely compatible with the media you're playing. If JMP or any client reports it isn't compatible for whatever reason, that triggers transcoding, remuxing, or transcoding of audio.